Quantitative analysis of various physiological indexes in wild-type and transgenic plants. (A,B) Cu content in wild-type and transgenic plant shoots and roots treated with 50 μM copper chloride (CuCl2) for 21 days, respectively. (C) abscisic acid (ABA), (D) malondialdehyde (MDA), and (E) hydrogen peroxide (H2O2) content in wild-type and transgenic plants treated with or without 50 μM CuCl2 for 24 h. (F,G) Chlorophyll and carotenoid content in wild-type and transgenic plants treated with or without 50 μM CuCl2 for 21 days. Values are means ± standard deviation (SD) of three independent biological replicates; * p < 0.05 and ** p < 0.01 indicate significant differences from wild-type plants under the same conditions determined using Student’s t-test.