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. 2019 Dec 2;20(23):6082. doi: 10.3390/ijms20236082

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Indirect immunofluorescence analysis of differentially expressed proteins. Expression of Rack1, ascertained by immunofluorescence in representative (A) normal and (B) tumor frozen tissue samples included in this study as well as by immunohistochemistry of (C) normal and (D) tumor tissues (images retrieved from human protein atlas HPA; www.proteinatlas.org/pathology, last accessed 03.03.2019), was examined to ascertain the differential cell type-specific expression of this candidate biomarker. Magnification is provided in each case by scale bars (100 µm and 40 µm).