Figure 6.

Proliferation and dead analysis on GBM and astrocytes in 3D co-culture. (A) Viability of mono- and co-cultures of GBM and astrocytes in the hyaluronic acid hydrogel (HyStem™-HP) compared to 2D cultures. Viability was obtained by a Cell Titer 96^® Cell Proliferation assay after 3 days of culture. (B) Propidium iodide positive cells (%) of GBM cells in mono- or co-culture with astrocytes in a 3D system was obtained by counting the number of propidium iodide (PI) negative GBM cells. (C) Proliferation of GBM and astrocytes in mono- or co-culture represented by CFSE-positive GBM and Far Red-positive astrocytes fluorescence. Mean ± SEM (n = 3). * p< 0.01, ** p < 0.05 in comparison to monoculture of GBM.