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. 2019 Nov 29;20(23):6017. doi: 10.3390/ijms20236017

Figure 7.

Figure 7

Drug response of GBM cells to TMZ, CLM, and VCR in 2D and 3D culture mono- or co-culture with astrocytes. (A1–A3) Viability of UP-007 cells when treated with TMZ (A1), CLM (A2), or VCR (A3) obtained by Cell Titer 96® Cell Proliferation assay. Cells were cultured either in 2D or 3D conditions (HyStem™-HP) alone or in co-culture with astrocytes at ratio 50:50. Cell viability was normalized with untreated cells for each of the models used (2D/3D and mono/co-culture). Mean ± SEM (n = 3). * p < 0.05, ** p < 0.05, *** p< 0.001, **** p < 0.0001 compared to untreated cells. Not significant (ns) p> 0.05, ## p < 0.05, ### p < 0.001, #### p < 0.001. (B1–B3) Corrected total cell fluorescence of GBM cultured in 3D treated with TMZ (400 µM) (B1), VCR (2 µM) (B2), or CLM (20 µM) (B3) either in mono- or co-culture with astrocytes. Mean fluorescence intensity (MIF) is inversely related to proliferation. Mean ± SEM (n = 3). * p < 0.05, ** p < 0.01 comparison to monoculture treated with the TMZ, CLM, or VCR.