Figure 9.

Confocal imaging shows homo-cellular and hetero-cellular extensions in 3D co-culture models using hyaluronic acid-based hydrogel and mitochondria co-localization in TNT formation. (A) CFSE-positive GBM (blue) cells were grown with astrocytes in HyStem™-HP hydrogel for three days and stained with WGA AF594 (red). TNTs connecting GBM cells and astrocytes. (B) Confocal imaging of TNTs in a co-culture system. Astrocytic cells were stained with Mito Tracker Orange FM, mixed 1/1 with CFSE GBM cells and subsequently embedded in HyStem™-HP hydrogel. Before imaging, membranes of the co-cultured cells were stained with WGA AF594. Scale bar: 50 µm.