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. 2016 Jan 4;74(3):ftv129. doi: 10.1093/femspd/ftv129

Figure 3.

Figure 3.

PCR detection of Chlamydiae in Peromyscus spp. Panels are representative results of PCR from selected tissues targeting amplification of Chlamydia 16s rRNA, Chlamydia genus-specific regions of ompA (ompA-1) and C. muridarum allele-specific ompA (ompA-2) genes. Diluted Chlamydia ompA-1 PCR product was used as template in the C. muridarum specific ompA-2 PCRs. Products shown were resolved on 1.5% agarose gels with ethidium bromide staining. PCR products of the same targets for C. muridarum genomic DNA (MoPn EB) were used for positive controls. Top panel is from a single male mouse. Bottom panel is from a single pregnant female mouse. Summary results are shown in the table below the panels. ILN: iliac lymph nodes. MLN: mesenteric lymph nodes.