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. 2019 Nov 20;20(23):5832. doi: 10.3390/ijms20235832

Figure 4.

Figure 4

Hyperoside activates nuclear translocation of Nrf2 in SH-SY5Y cells. Hyperoside-induced Nrf2 activation is of a concentration- (A) and time-dependent (B) manner in SH-SY5Y cells. Cells were treated with hyperoside at the indicated times (0–12 h) and concentrations (0.1–2 μM), after which nuclear fractions were prepared to analyze the distribution of Nrf2. Levels of Nrf2 and laminin B1 were evaluated by Western blot analysis. Cells were transiently transfected with an ARE reporter plasmid construct and then treated with the indicated concentrations of hyperoside for 4 h. Equal amounts of cell extracts were assayed for dual-luciferase activity (C). Nrf2 was detected using an anti-Nrf2 antibody and a Texas red®-conjugated secondary antibody. Nuclei were stained with Hoechst 33258. Images shown are representative of three experiments and were captured using a fluorescence microscope (D, 100× magnification). Error bars indicate the mean ± SEM (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control group. Scale bar: 50 μm.