Fig. 7.

Inhibition of autophagy triggers melatonin-induced apoptosis in glioblastoma cells. a U87MG cells. b A172 cells. The cells were treated with DMSO as a negative control, 3-MA (10 mM), or melatonin (1 mM), or were co-treated with 3-MA and melatonin for 24 h. The 3-MA was added to the medium 1 h prior to the administration of melatonin. Cell death was determined by flow cytometry following Annexin V/PI staining. The percentages of cells in early and late apoptosis and the total number of apoptotic cells are presented as the mean ± S.D. from three independent experiments. Statistical significance *P < 0.01 vs controls or the corresponding group