Table 2.
Additional primers used for the detection of Wolbachia and other symbionts
| Species/Gene marker | Primer | Primer sequences (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
| Wolbachia/groE | WgroF1 | GGTGAGCAGTTGCAAGAAGC | 846 |
| Wgro Rev1 | AGATCTTCCATCTTGATTCC | ||
| Wolbachia/gltA | WgltAF1 | TACGATCCAGGGTTTGTTTCTAC | 554 |
| WgltARev1 | CTCATTAGCTCCACCGTGTG | ||
| Wolbachia/dnaA | dnaA2F | ACAATTGGTTATATCAGCTG | 378 |
| dnaA2R | TACATAGCTATTTGYCTTGG | ||
| Wolbachia/aspC | aspC49_F | ATYGCTGTRACYGATAAGGYAA | 818 |
| aspC1134R | AGARGTWGCATAAGARATTCTRA | ||
| Wolbachia/atpD | atpD242F | ATAYAGTKCGTTGTATTGCTATG | 881 |
| atpD1210R | CWTCAGAYAGYTCATCCATAC | ||
| Wolbachia/sucB | sucB358F | AAAGGRACTGGYATGGGARG | 612 |
| sucB981R | TGHGGAGGRTTWATWATCGG | ||
| Wolbachia/pdhB | pdhB86F | ARGAAGTTGCVGARTATSAWGG | 642 |
| pdhB812R | GCAAAWRRCCAWCCTTCTTCTA | ||
| Spiroplasma sp./16S rRNA | SpoulF | GCTTAACTCCAGTTCGCC | 421 |
| SpoulR | CCTGTCTCAATGTTAACCTC | ||
| Cardinium sp./16S rRNA | Ch-F | TACTGTAAGAATAAGCACCGGC | 463 |
| Ch-R | GTGGATCACTTAACGCTTTCG | ||
| Rickettsia sp./gltA | RicCS-AF | TTTAGGCTAATGGGTTTTGGTCAT | 382 |
| RicCS-AR | TGCCCAAGTTCTTTTAACACCTC | ||
| Arsenophonus sp./16S rRNA | ArsF | GGGTTGTAAAGTACTTTCAGTCGT | 581–804 |
| ArsR2 | GTAGCCCTRCTCGTAAGGGCC | ||
| Hamiltonella sp./16S rRNA | HamF | TGAGTAAAGTCTGGAATCTGG | 700 |
| HamR | AGTTCAAGACCGCAACCTC |