Fig. 3.

Verification of circular RNA (circRNA) data from RNA sequencing. a Divergent primers to amplify the circular junctions. Red arrows represent divergent primers. b Reverse transcription PCR amplification of circRNA with divergent primers. The PCR products of circRNA2185, circRNA2184, circRNA681, circRNA5146, circRNA597, and circRNA2554 were analyzed using gel electrophoresis. M is the marker and N is the negative control. c Head-to-tail splice junctions were confirmed using DNA sequencing