Table 3.
Clinical and biological presentation of the 4 BPDCN cases expressing a combination of lymphoid markers (B and T) plus myeloid markers
| Patients | P101 | P1506 | P1603 | P201 |
|---|---|---|---|---|
| Age, y | 76 | 66 | 69 | 81 |
| T-cell markers | ||||
| CD2 | − | − | − | + |
| CD5 | − | − | − | − |
| CD7 | + | + | + | − |
| CD3 | − | − | − | − |
| cCD3 | − | − | − | − |
| B-cell markers | ||||
| CD22 | − | + | + | + |
| CD79a | + | − | − | − |
| cCD22 | − | ND | ND | ND |
| CD19 | − | − | − | − |
| Myeloid markers | ||||
| CD13 | − | − | − | − |
| CD117 | + | − | − | − |
| CD15 | − | − | − | − |
| CD65 | − | − | − | − |
| CD33 | + | + | + | + |
| CD38 | + | + | ND | ND |
| CD36 | + | + | + | + |
| MPO | − | − | − | − |
| pDC markers | ||||
| HLA-DR | + | + | + | + |
| CD4 | + | + | + | + |
| CD56 | + | + | + | + |
| CD123 | + | + | + | + |
| CD303 | ND | + | ND | + |
| CD304 | ND | + | ND | + |
| cTCL1 | ND | + | + | ND |
| Karyotype | Failure | Normal | Normal | Normal |
| Bone marrow aspirate | ||||
| Dysplasia | No | Dysgranulopoiesis | Dysgranulopoiesis | Trilineage dysplasia |
| Blast morphology | Large pseudopodia, rare vacuoles, undifferentiated cells | Medium size, some vacuoles, immature | Typical with vacuoles and pseudopodia | Typical with vacuoles and pseudopodia |
| Cutaneous lesions | Extensive petechiae skin purpura, from thighs to neck | Multiple brown lesions on the back and trunk | Purplish nodular lesions on the forehead and scalp | Diffuse ecchymotic lesions on the trunk, face, upper and lower limbs |
| Immunohistochemistry | CD4+ CD56+ MPO− CD3− CD20− CD138− immunoglobulin− | CD4+ CD56+ CD3− CD20− MPO− | CD123+ CD4+ CD56+ CD45RA+ CD34− MPO− TDT− CD15− CD3− CD2− CD8− CD20− | CD4+ CD56+ CD68+ CD123+ CD303+ TCL1+ CD2AP+ |
c, intracytoplasmic detection; ND, not done.