Skip to main content
. 2019 Dec 19;3(24):4187–4201. doi: 10.1182/bloodadvances.2019000531

Figure 3.

Figure 3.

Vit C–treated CD8+ iTregs enhance Foxp3 stability during GVHD development. (A) Experimental scheme: lethally irradiated BALB/c mice were adoptively transferred with 5 × 106 Rag1−/− BM cells and 1 × 106 CD8+ iTregs (Ly5.2+). Three days later, 2 × 106 CD25-depleted T cells from C57BL/6 (Ly5.1+) congenic mice were injected IV. On day 7 after allogeneic BMT, spleen, liver, and mesenteric lymph nodes (mLNs) were harvested and analyzed. (B) Foxp3+ retention by transferred control and Vit C–treated CD8+ iTregs. (C) IFN-γ expression of transferred CD8+ iTregs. Data are combined from 2 independent experiments (n = 7 per group). *P ≤ .05, **P ≤ .01, ***P ≤ .001, ****P ≤ .0001, Student t test.