Table 4.
Summary of internal evaluations for each compared methods
| Datasets | Methods | Internal evaluations | ||
|---|---|---|---|---|
| CH | DB | XB | ||
| Cell Cycle | Accense | 3.2409 ± 0.2069 | 2.4746 ± 0.2563 | 0.6473 ± 0.0750 |
| PhenoGraph | 3.4250 ± 0.0350 | 2.5692 ± 0.1503 | 0.7864 ± 0.0392 | |
| Xshift | 3.5620 ± 0.0443 | 1.7337 ± 0.1618 | 0.9131 ± 0.3066 | |
| kmeans | 3.9414 ± 0.0011 | 1.5971 ± 0.0013 | 0.7705 ± 0.0165 | |
| flowMeans | 3.5992 ± 0.0283 | 1.6237 ± 0.0341 | 0.7294 ± 0.0393 | |
| FlowSOM | 3.6294 ± 0.0036 | 1.3578 ± 0.0351 | 0.6669 ± 0.0269 | |
| DEPECHE | 3.8372 ± 0.0283 | 1.7767 ± 0.0322 | 0.7695 ± 0.0177 | |
| ACDC | 3.4645 ± 0.0901 | 2.0773 ± 0.3156 | 0.9395 ± 0.0428 | |
| LDA | 3.4581 ± 0.0036 | 2.5080 ± 0.0191 | 1.0021 ± 0.0358 | |
| Colon | Accense | 3.2688 ± 0.0622 | 1.7064 ± 0.0656 | 1.0427 ± 0.0545 |
| PhenoGraph | 3.4231 ± 0.0194 | 1.9030 ± 0.0245 | 1.3997 ± 0.1199 | |
| Xshift | 3.0706 ± 0.0179 | 2.3218 ± 0.0595 | 1.3207 ± 0.0511 | |
| kmeans | 3.5109 ± 0.0079 | 1.9037 ± 0.0501 | 1.4570 ± 0.1055 | |
| flowMeans | 3.4793 ± 0.1068 | 1.5864 ± 0.0421 | 1.6706 ± 0.1958 | |
| FlowSOM | 3.6435 ± 0.0117 | 1.6583 ± 0.0514 | 1.5910 ± 0.1568 | |
| DEPECHE | 3.9819 ± 0.0375 | 1.7725 ± 0.0250 | 1.6325 ± 0.0930 | |
| ACDC | 3.5898 ± 0.0440 | 2.0607 ± 0.1160 | 1.4239 ± 0.0754 | |
| LDA | 3.5100 ± 0.0009 | 2.1773 ± 0.0065 | 1.5564 ± 0.0724 | |
| Levine13dim | Accense | 3.4230 ± 0.0932 | 1.8832 ± 0.1408 | 1.2321 ± 0.0278 |
| PhenoGraph | 4.0739 ± 0.0176 | 1.4645 ± 0.0346 | 1.3972 ± 0.1349 | |
| Xshift | 3.5106 ± 0.0289 | 2.4284 ± 0.0443 | 1.7868 ± 0.0476 | |
| kmeans | 3.8508 ± 0.0150 | 2.1550 ± 0.0546 | 1.6213 ± 0.1471 | |
| flowMeans | 4.0475 ± 0.0194 | 1.5030 ± 0.0849 | 1.4234 ± 0.1182 | |
| FlowSOM | 3.8486 ± 0.0071 | 1.7564 ± 0.0615 | 1.5043 ± 0.1531 | |
| DEPECHE | 4.2783 ± 0.0174 | 1.1677 ± 0.0342 | 1.3562 ± 0.0392 | |
| ACDC | 3.9638 ± 0.0110 | 1.4916 ± 0.0370 | 1.3109 ± 0.0948 | |
| LDA | 3.8288 ± 0.0106 | 2.0046 ± 0.0493 | 1.3828 ± 0.1167 | |
| Levine32dim | Accense | 3.4621 ± 0.0901 | 2.3414 ± 0.0925 | 0.7891 ± 0.0950 |
| PhenoGraph | 3.7401 ± 0.0081 | 1.8293 ± 0.0810 | 1.0009 ± 0.0479 | |
| Xshift | 3.6669 ± 0.0102 | 2.2576 ± 0.1324 | 0.8295 ± 0.1382 | |
| kmeans | 3.8761 ± 0.0166 | 2.0587 ± 0.0386 | 0.9972 ± 0.0441 | |
| flowMeans | 3.8546 ± 0.0393 | 1.6975 ± 0.2199 | 0.7985 ± 0.0709 | |
| FlowSOM | 3.8244 ± 0.0285 | 1.5974 ± 0.0863 | 0.8366 ± 0.0792 | |
| DEPECHE | 4.1480 ± 0.0009 | 1.4727 ± 0.0023 | 0.7575 ± 0.0351 | |
| ACDC | 3.6169 ± 0.0046 | 1.3974 ± 0.0049 | 0.7693 ± 0.1310 | |
| LDA | 3.8297 ± 0.0007 | 1.7011 ± 0.0099 | 0.7155 ± 0.0139 | |
| Muscle | Accense | 3.2254 ± 0.1688 | 2.3190 ± 0.3178 | 0.8420 ± 0.1211 |
| PhenoGraph | 3.6052 ± 0.0132 | 1.8619 ± 0.0417 | 1.7228 ± 0.2389 | |
| Xshift | 3.2898 ± 0.0274 | 2.2460 ± 0.1524 | 2.1455 ± 0.1599 | |
| kmeans | 3.9722 ± 0.0022 | 1.7729 ± 0.0099 | 1.4987 ± 0.1212 | |
| flowMeans | 3.3809 ± 0.0565 | 1.7685 ± 0.0769 | 1.3750 ± 0.1428 | |
| FlowSOM | 3.8262 ± 0.0146 | 1.4439 ± 0.0462 | 1.3586 ± 0.1473 | |
| DEPECHE | 4.2639 ± 0.0044 | 1.2235 ± 0.0080 | 1.2892 ± 0.0269 | |
| ACDC | 3.6900 ± 0.0305 | 1.7186 ± 0.0515 | 1.5641 ± 0.15555 | |
| LDA | 3.8073 ± 0.0031 | 1.6641 ± 0.0206 | 1.6003 ± 0.0900 | |
| Samusik01 | Accense | 3.2952 ± 0.0476 | 2.3424 ± 0.1454 | 0.6500 ± 0.0266 |
| PhenoGraph | 3.7380 ± 0.0033 | 1.4607 ± 0.0424 | 0.7669 ± 0.0831 | |
| Xshift | 3.2660 ± 0.0134 | 2.7855 ± 0.4644 | 1.2670 ± 0.1654 | |
| kmeans | 3.7087 ± 0.0135 | 2.2072 ± 0.0854 | 0.9365 ± 0.0792 | |
| flowMeans | 3.4029 ± 0.0381 | 1.6069 ± 0.2374 | 0.6309 ± 0.0475 | |
| FlowSOM | 3.6941 ± 0.0257 | 1.8967 ± 0.3799 | 0.8286 ± 0.0832 | |
| DEPECHE | 4.1028 ± 0.0021 | 1.4867 ± 0.0054 | 0.9141 ± 0.0109 | |
| ACDC | 3.6827 ± 0.0178 | 1.3871 ± 0.0132 | 0.7273 ± 0.0573 | |
| LDA | 3.6767 ± 0.0080 | 1.6325 ± 0.0242 | 1.0414 ± 0.0808 | |
Data shown as mean ± standard deviation. CH Calinski-Harabasz index (log10 transformed), DB Davies-Bouldin index, XB Xie-Beni index (log10 transformed)