Table 1.

Effect of Fe₃O₄@SiO₂–NH₂ nanoparticle administration on blood biochemical indexes

Indexes	C6a	S6	M6	L6	C48	S48	M48	L48
TP (g/L)	54.96 ± 2.18b	55.44 ± 2.29	57.56 ± 3.38	57.62 ± 1.60	55.53 ± 1.98	56.71 ± 2.36	55.04 ± 2.26	56.30 ± 3.59
Alb (g/L)	30.66 ± 1.01	30.54 ± 0.74	30.98 ± 1.08	30.74 ± 0.35	30.95 ± 0.75	30.86 ± 0.88	29.81 ± 0.75*	30.51 ± 1.25
Glo (g/L)	24.30 ± 1.24	24.90 ± 1.69	26.58 ± 2.37	26.88 ± 1.35*	24.58 ± 1.43	25.85 ± 1.62	25.23 ± 1.63	25.79 ± 2.36
Alb/Glo	1.26 ± 0.03	1.23 ± 0.06	1.17 ± 0.07*	1.15 ± 0.05**	1.26 ± 0.06	1.2 ± 0.05	1.19 ± 0.05*	1.19 ± 0.06
Tbil (μmol/L)	2.14 ± 0.23	2.36 ± 0.15	2.06 ± 0.25	2.24 ± 0.45	2.22 ± 0.19	2.40 ± 0.21	2.39 ± 0.16	2.39 ± 0.21
Dbil (μmol/L)	0.80 ± 0.07	0.72 ± 0.08	0.78 ± 0.11	0.78 ± 0.08	0.82 ± 0.08	0.86 ± 0.09	0.85 ± 0.09	0.88 ± 0.10
Ibil (μmol/L)	1.34 ± 0.18	1.64 ± 0.09*	1.28 ± 0.25	1.46 ± 0.40	1.40 ± 0.15	1.54 ± 0.14	1.54 ± 0.09	1.51 ± 0.12
ALT (U/L)	56.20 ± 5.81	51.20 ± 5.36	65.80 ± 8.41	76.80 ± 33.07	49.17 ± 8.86	46.88 ± 6.33	48.13 ± 9.72	45.88 ± 3.40
AST (U/L)	204.40 ± 36.05	195.20 ± 33.52	202.60 ± 18.94	195.60 ± 60.80	159.50 ± 45.05	139.25 ± 33.78	135.25 ± 26.76	133.75 ± 23.87
AST/ALT	3.64 ± 0.51	3.88 ± 0.93	3.10 ± 0.35	2.66 ± 0.40*	3.23 ± 0.59	2.96 ± 0.47	2.85 ± 0.45	2.91 ± 0.46
GGT (U/L)	0.64 ± 0.49	0.28 ± 0.40	0.28 ± 0.40	0.46 ± 0.49	1.00 ± 0.00	0.55 ± 0.48	0.89 ± 0.32	1.00 ± 0.00
ALP (U/L)	414.00 ± 119.44	338.00 ± 38.86	353.60 ± 20.79	345.20 ± 34.54	363.33 ± 77.29	320.5 ± 68.26	308.75 ± 61.68	291.38 ± 46.15
TG (mmol/L)	1.53 ± 0.35	1.49 ± 0.40	1.73 ± 0.65	2.23 ± 1.88	1.77 ± 0.62	1.76 ± 0.65	1.16 ± 0.33	0.91 ± 0.25**
TC (mmol/L)	2.49 ± 0.36	2.25 ± 0.42	2.54 ± 0.43	3.06 ± 0.35*	2.19 ± 0.18	2.42 ± 0.28	2.24 ± 0.25	2.48 ± 0.22**
HDL-C (mmol/L)	0.78 ± 0.10	0.68 ± 0.14	0.73 ± 0.09	0.80 ± 0.22	0.71 ± 0.07	0.75 ± 0.10	0.69 ± 0.11	0.77 ± 0.07
LDL-C (mmol/L)	0.27 ± 0.05	0.26 ± 0.07	0.34 ± 0.13	0.48 ± 0.10**	0.20 ± 0.03	0.25 ± 0.04*	0.26 ± 0.03*	0.25 ± 0.05
Glc (mmol/L)	5.19 ± 0.41	5.94 ± 0.26**	5.28 ± 0.77	5.93 ± 0.37*	5.64 ± 0.68	5.46 ± 0.50	6.03 ± 0.61	6.00 ± 0.37
LDH (U/L)	1436.50 ± 141.95	1243.36 ± 255.38	910.08 ± 165.81**	683.84 ± 202.51**	1143.87 ± 415.51	1007.39 ± 324.97	790.78 ± 253.54	749.79 ± 285.97
BUN (mmol/L)	4.88 ± 0.86	4.74 ± 0.93	4.00 ± 0.64	5.01 ± 0.60	4.76 ± 0.62	4.92 ± 0.39	5.70 ± 0.52*	4.96 ± 0.69
Cn (μmol/L)	46.60 ± 1.14	45.40 ± 4.83	44.60 ± 0.55**	45.20 ± 1.92	44.67 ± 1.97	44.00 ± 1.85	43.75 ± 2.25	45.75 ± 2.66
BUN/Cn	0.11 ± 0.02	0.11 ± 0.01	0.09 ± 0.02	0.11 ± 0.02	0.11 ± 0.02	0.11 ± 0.01	0.13 ± 0.01*	0.11 ± 0.02
UA (μmol/L)	89.00 ± 15.81	124.60 ± 8.68**	145.60 ± 14.93**	154.40 ± 17.14**	96.17 ± 17.01	111.75 ± 18.97	132.88 ± 14.26**	122.25 ± 25.79*
TBA (μmol/L)	21.78 ± 7.62	21.44 ± 5.74	18.82 ± 5.01	30.98 ± 7.49	21.97 ± 7.26	24.01 ± 6.79	28.9 ± 19.12	23.93 ± 9.87

TP total protein, Alb albumin, Glo globulin, Tbil total bilirubin, Dbil direct bilirubin, Ibil indirect bilirubin, ALT alanine aminotransferase, AST aspartate aminotransferase, GGT gamma glutamyltransferase, ALP alkaline phosphatase, TG triglycerides, TC total cholesterol, HDL high-density lipoprotein, LDL low-density lipoprotein, Glc glucose, LDH lactate dehydrogenase, BUN blood urea nitrogen, Cn creatinine, UA uric acid, TBA total bile acid

* Significant differences between treatment and control groups as judged by Student’s t-test using SPSS (*P < 0.05; **P < 0.001)

^aC, control group; S, small-size group (around 10 nm in diameter); M, mid-size group (around 20 nm in diameter); L, large-size group (around 40 nm in diameter); 6, 6 h post-dose; 48,48 h post-dose

^bEach value data represents the mean ± S.D