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. 2019 Dec 23;21:298. doi: 10.1186/s13075-019-2075-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Binding of PlGF-2_123-144-α-TNF to human cartilage. Unmodified α-TNF and PlGF-2_123-144-α-TNF binding to fibronectin (Fn), decorin (DCN), collagen I (Col I), Col II, Col III, and Col IV were analyzed by ELISA (n = 3, mean + SE). *P < 0.05, compared with binding of unmodified α-TNF (Student’s t test). b Representative images of human osteoarthritis specimen probed with either unmodified α-TNF and PlGF-2_123-144-α-TNF (red), anti-type II collagen antibody (blue), and anti-decorin antibody (green). Scale bar, 500 μm