Figure 5.

miR-429 controls HER2+ BC cell proliferation , migration and invasion. MTT assay was performed on SKBR-3 cell at 0, 24, 48 h after 200nM Scramble or As miR-429 oligonucleotide treatment. The results are the average of three independent experiments in triplicate (t test compared to scramble treated cells, p value <0.05, *; <0.01, **)(A). 200nM 72h treatment with As miR-429 reduced the expression of CDK4 significantly (t test n=2 experiments in triplicate, p value <0.01**)(B). Wound healing test was performed on SKBR-3 treated with 200nM scramble oligonucleotide or As miR-429 in culture for 9 days. In each sample, the wound area was quantified by Image J following ³¹(t test compared to scramble-treated cells; n=3 experiments in triplicate, p value <0.01, **) (C). Boyden's chamber test was performed on SKBR-3 in the presence of 150nM scramble or As miR-429 in culture for 24h. The count of the cells was performed on 10 images from each sample (t test compared to scramble-treated cells; n=3 experiments, p value<0.01, **) (D).