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. 2020 Jan 1;10(1):50–61. doi: 10.7150/thno.36274

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VHL is the direct target of miR-429. Luciferase assay: HER2+ cells were transfected with the Luciferase+ 3'UTR VHL (pGL3-VHL) construct alone, with 100nM 48h S Scramble or S miR-142 or As miR-429. The luciferase expression (LUC) was normalized on alkaline phosphatase content (AP). The chart represents the percentage of LUC/AP ratio (T test, p value<0.05, *; n=2 independent experiment)(A). RT-PCR analysis revealed that miR-429 modulation, obtained with 50nM As miR-429 for 72h (B), caused a significant increase of VHL expression, both at mRNA (C) and protein levels (D) (T test, p value<0.05, *; n=3 independent experiment). RT-PCR analysis on human HER2+ BC samples revealed that miR-429 is downregulated, compared to healthy tissue (E).