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. 2020 Jan 1;10(1):17–35. doi: 10.7150/thno.36930

Figure 9.

Figure 9

Substitution of MSR1 KO bone marrow with MSR1 WT bone marrow promotes intramembranous bone healing. (A) Schematic representation of the main steps of the bone marrow transplant. (B) Representation of 3D images of injured tibiae from different transplanted mice (KO to KO vs. WT to KO) by micro-CT on day 7 or 14 post-surgery. (C-F) Quantification of BV/TV (%) (C), Tb. Th (mm) (D), Tb. N (/mm) (E) and Tb. Sp (mm) (F) in the defect region on day 7 and 14 post-surgery for different transplanted mice (KO to KO vs. WT to KO) (Values are expressed as mean ± SD, *p < 0.05). (G) Representative IF images of total macrophages (F4/80+), M1-like macrophages (iNOS+ F4/80+) and M2-like macrophages (CD206+ F4/80+) in facture tissues from the bone marrow of transplanted mice (transplanted MSR1 KO bone marrow to host KO mice and transplanted MSR1 WT bone marrow to host KO mice) on day 7 post-surgery in the tibial monocortical defect model. Nuclei were counterstained with DAPI (blue). Bar = 100 μm. (H) The iNOS+ and CD206+ macrophage fractions were determined from different transplanted mice (KO to KO vs. WT to KO) on day 7 post-surgery of the tibial monocortical defect model (Values are expressed as mean ± SD, *p < 0.05, **p < 0.01). (I) mRNA expression levels of macrophage marker genes (M1-like: iNOS and IL-1b, M2-like: CD206 and CD163) in fracture tissues from different transplanted mice on day 7 in the tibial monocortical defect model (K) (*p < 0.05, **p < 0.01, ***p < 0.001).