Figure 7.

Peblr20 induces DNA demethylation at the Pou5F1 enhancer loci. (A) CpG islands at the Pou5F1 locus. CpG1: 5'-distal enhancer (5'-DE); CpG2:5' proximal enhancer (5'-PE); CpG3: promoter region (P); CpG4: 3'-enhancer (3'-Enh). (B) Peblr20 induces CpG demethylation at the Pou5F1 locus. DNAs were extracted from fibroblasts from gain-of-function experiments, treated by sodium bisulfite, amplified with the Pou5F1-specific primers, cloned into the pJET vector, and sequenced. Solid circle: methylated CpG; open circle: un-methylated CpG. Each line represents sequencing from one clone. Number under the last line represents the methylation level of each CpG island.