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. 2020 Jan 1;10(1):353–370. doi: 10.7150/thno.39093

Figure 8.

Figure 8

Peblr20 recruits TET2 to the Pou5F1 enhancer loci. (A) Interaction of TET2 at the Pou5F1 enhancer loci in the Peblr20 overexpressing fibroblasts. Enrichments of TET2 to the Pou5F1 enhancers were measured from TET2 ChIP samples and normalized to the IgG control samples. Compared to vector control group, there were remarkable enrichments of Pou5F1 enhancer loci with TET2 in Peblr20 overexpressed fibroblasts. 5'-DE: 5'-distal enhancer; 5'-PE: 5'-proximal enhancer; 3'E: 3'-enhancer. (B) No binding of TET1 with Pou5F1 enhancer loci. Fold enrichments of Pou5F1 enhancer loci were measured using TET1 ChIP samples relative to IgG samples. (C) Peblr20 recruits TET2. Fibroblasts transfected with Peblr20 were collected and immunoprecipitated with anti-TET2 antibodies, with mouse IgG as negative control and with anti-SNRNP70 as positive control. Purified binding RNAs were reverse transcribed and used as Q-PCR templates. RIP data are represented by fold enrichments relative to the input. (D) RIP data represented by fold enrichments relative to IgG showed that TET2 interacted primarily with the 3'- and 5'-fragment of Peblr20 lncRNA.