Skip to main content
. 2020 Jan 1;10(1):340–352. doi: 10.7150/thno.39066

Figure 5.

Figure 5

Nuclear-cytoplasmic shuttling of CREB, CRTC1 or SOX10. (A) Western blot analysis (WB) of CREB, CRTC1 or SOX10. B16-F0 cells were pretreated with BPT for 2 h and stimulated with SCF for 1 h in the presence of BPT. Cell extracts were partitioned between the cytosol and the nucleus. (B, C) WB on the phosphorylation of CREB or the dephosphorylation of CRTC1. B16-F0 cells were pretreated with BPT for 2 h and stimulated with SCF for indicated time points in the presence of BPT. (D) RT-PCR analysis on the induction of SOX10. B16-F0 cells were pretreated with BPT for 2 h and stimulated with SCF for indicated time points in the presence of BPT. Data are mean ± SEM. #P < 0.05 vs. medium alone. *P < 0.05 vs. SCF alone.