Figure 6.

DNA binding ability of CREB or SOX10 at the MITF-M promoter. Chromatin immunoprecipitation assay (ChIP). B16-F0 cells were pretreated with BPT for 2 h and stimulated with SCF for 1 h in the presence of BPT. Chromatin fragments were precipitated with anti-CREB (A) or anti-SOX10 antibody (B). Input and precipitated DNAs were subjected to PCR encompassing CREB- (A) or SOX10-responsive cis-acting element (B) at the MITF-M promoter. Data are mean ± SEM. ^#P < 0.05 vs. medium alone. *P < 0.05 vs. SCF alone. (C) A proposed model of SCF/KIT-activated transcription factors at the MITF-M promoter.