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. 2020 Jan 1;10(1):323–339. doi: 10.7150/thno.38870

Figure 1.

Figure 1

F. nucleatum is Associated with Colorectal Cancer Metastasis. (A) A cladogram representation of by 16S rDNA sequencing data from CRC patients with (n = 9) and without (n = 7) metastasis. Taxa enriched in patients with metastasis (red) and without metastasis (green). The brightness of each dot is proportional to its effect size. (B) Linear discriminant analysis (LDA) coupled with effect size measurements identified the significantly differential abundances in the data referenced in A. Only taxa with values greater than the LDA threshold of 3.6 are shown. (C) Statistical analysis of the abundance of F. nucleatum in CRC patients with or without metastasis (**P < 0.01, and ***P < 0.001; nonparametric Mann-Whitney test). (D) Representative images of FISH to assess the amount of F. nucleatum in metastatic CRC tissues (n = 62), non-metastatic CRC tissues (n = 32) and matched lymph nodes. EUB338 (red) is a Cy3-conjugated “universal bacterial” oligonucleotide probe; FUS664 (green) is a FITC-conjugated F. nucleatum oligonucleotide probe. 200× magnification. (E-H) Statistical analysis of the mRNA expression of E-cadherin and Vimentin in CRC patients with or without metastasis (E-F; *P < 0.05, **P < 0.01, and ***P < 0.001; nonparametric Mann-Whitney test; the error bars indicate the SDs). Correlation analysis of the abundance of F. nucleatum and the expression levels of E-cadherin and Vimentin in cancer tissues (G-H; two-tailed, nonparametric Spearman correlation). (I-J) Western blot analysis was performed with CRC cells cocultured with F. nucleatum (F01), F. nucleatum (ATCC10951), E. coli or PBS (control).