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. 2020 Jan 1;10(1):323–339. doi: 10.7150/thno.38870

Figure 4.

Figure 4

CARD3 Expression is Associated with F. nucleatum.(A) GSEA for CARD3 expression with metastatic gene sets using the GSE21510 dataset (NES = -1.860, P = 0.0058, FDR = 0.055). (B-C) Statistical analysis of CARD3 mRNA expression in CRC tissues from patients with or without metastasis (B; ***P < 0.001; nonparametric Mann-Whitney test; the error bars indicate the SDs). Correlation analysis of the abundance of F.nucleatum and the expression of CARD3 in CRC tissues (C; r = 0.6839, P < 0.001; two-tailed, nonparametric Spearman correlation). (D-E) Representative images showing that the abundance of invasive F. nucleatum in CRC tissues is associated with CARD3 expression. 100× or 200× magnification (D). CARD3 protein levels in 21 F. nucleatum-low and 22 F. nucleatum-high CRC tissues from patients were measured by immunohistochemical analysis (E; *P < 0.05; unpaired Student's t-test; the error bars indicate the SDs). (F) Representative images of Western blots comparing the expression of CARD3 in F. nucleatum-low and F. nucleatum-high CRC tissues from patients. (G-I) Statistical analysis of CARD3 mRNA expression in HCT116 cells cocultured with F. nucleatum (F01, G). Western blot analysis was performed to measure CARD3 expression in HCT116 cells cocultured with F. nucleatum (F01) or E. coli in a time-dependent (H) or MOI-dependent (I) manner.