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. 2020 Jan 1;10(1):411–425. doi: 10.7150/thno.33482

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AD-MSCs were successfully engineered to secrete miR-101-enriched EVs. NC, negative control; AD-MSC, adipose tissue-derived mesenchymal stromal cell; CM-EV, EVs derived from conditioned media of AD-MSCs; NC-EV, EVs derived from AD-MSCs-NC; miR-101-EV, EVs derived from AD-MSCs-101. (A) Transduction efficiency of miR-101 was measured by qRT-PCR. Student's t-test. (B) Steps of differential centrifugation. (C) Representative TEM image of AD-MSC-derived EVs. (D) NTA results for AD-MSC-derived EVs (n=3). (E) Expression of surface markers of EVs by western blotting. Equal amounts (2 μg) of protein were loaded in each lane, and AD-MSC lysate was used as a control for EV characterization. (F) Relative amounts of miR-101 in AD-MSC-101-EVs and AD-MSC-NC-EVs. Student's t-test. ^***P<0.001.