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. 2020 Jan 1;10(1):411–425. doi: 10.7150/thno.33482

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AD-MSC-101-EVs suppressed the invasion and migration abilities of osteosarcoma cells. NC-EV, EVs derived from AD-MSCs-NC; miR-101-EV, EVs derived from AD-MSCs-101. (A-B) Uptake of miR-101-EVs by 143B and Saos-2 cells as observed using confocal microscopy (magnification, 400×). (C-D) Effect of AD-MSC-101-EVs on BCL6 mRNA and protein expression as determined by qRT-PCR and western blotting, respectively, in 143B and Saos-2 cells. One-way ANOVA and Tukey's multiple comparisons test. (E-F) Effect of AD-MSC-101-EVs on invasion and migration of 143B and Saos-2 cells in a Transwell assay (magnification, 200×). One-way ANOVA and Tukey's multiple comparisons test. (G-H) Effect of AD-MSC-101-EVs on the migration of 143B and Saos-2 cells as observed in a wound healing assay (magnification, 40×). One-way ANOVA and Tukey's multiple comparisons test. ^**P<0.01 and ^***P<0.001 compared with PBS or NC-EV treatment.