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. 2020 Jan 1;10(1):300–311. doi: 10.7150/thno.38736

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Characterization of the native liver, DLM, and Young's modulus of DLM/GelMA hydrogel. (A) Gross images of a rat liver and DLM. (B) H&E staining of a native liver before and after decellularization. (C) SEM characterization of a native before and after decellularization. (D-G) Immuno-characterization of collagen I (red), collagen IV (red), fibronectin (red), and laminin (red) in the native liver and DLM. Nuclei of the cells were counterstained with DAPI (blue). (H) Measurement of Young's modulus of 2:3 DLM/GelMA hydrogel with varying cell ratios. Scale bar: 100 μm (B, D-G). (Avg. ± SD, NS indicates p > 0.05, n = 3).