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Evaluation of 5-FU-loaded PLGA-PEG NPs' toxicity using the metastatic tumor progression model. (A) Live/dead staining of HepLL and Caki-1 cells as well as the effects of 5-FU and 5-FU-loaded NPs on cell viability at 24 hours. (B-H) Cell viability of various ratios of HepLL to Caki-1 cells treated with 30 μg/mL of 5-FU or 5-FU-loaded NPs in the tumor progression model based on metastasis-on-a-chip at 24 hours. (Avg. ± SD, * stands for p < 0.05, n = 3).
