Table 2.
Identification of potential biomarkers of rat plasma samples between control group and chronic migraine module group.
| NO | Retention Time | Name | Formula | Experimental Mass | Ion Mode | Mass Error | MS/MS |
|---|---|---|---|---|---|---|---|
| P1 | 7.12 | omega-Muricholate | C24H40O5 | 407.2803 | [M-H]- | 1.7 | 389.2695, 346.2837, 289.2176, 209.1544, 195.1758 |
| P2 | 11.44 | Gymnodimine | C32H45NO4 | 506.3276 | [M-H]- | -4.3 | 488.3161 |
| P3 | 3.81 | 2-hydroxy-3-oxoadipic acid | C6H8O6 | 175.0248 | [M-H]- | -1.1 | 157.0135, 129.0194,113.0245, 85.0297, 59.0142 |
| P4 | 9.38 | Glycocholic acid | C26H43NO6 | 500.2784 | [M+Cl]- | -0.6 | 464.3020, 402.3008 |
| P5 | 19.47 | Petroselinic acid | C18H34O2 | 281.2486 | [M-H]- | -0.7 | 281.2486, 263.2380 |
| P6 | 21.27 | Stearic acid | C18H36O2 | 283.2637 | [M-H]- | -2.0 | 269.2487, 255.2329, 241.2180, 227.2017 |
| P7 | 21.51 | 11Z-Eicosenoic acid | C20H38O2 | 309.2799 | [M-H]- | -0.6 | 265.2895 |
| P8 | 9.34 | Isodeoxycholic acid | C24H40O4 | 391.2854 | [M-H]- | -0.5 | 373.2738, 329.2844 |
| P9 | 8.58 | sphinganine 1-phosphate | C18H40NO5P | 380.2571 | [M-H]- | -1.1 | 253.2170, 224.0692, 163.1127 |
| P10 | 2.65 | L-Tryptophan | C11H12N2O2 | 203.0826 | [M-H]- | -1.5 | 186.0568, 130.0666, 116.0510, 74.0254 |
| P11 | 0.58 | Lysine | C6H14N2O2 | 145.0982 | [M-H]- | -0.7 | 131.0826, 113.0732 |
| P12 | 0.73 | glutamic acid | C5H9NO4 | 146.0465 | [M-H]- | -1.6 | 128.0355, 102.0546 |
| P13 | 0.62 | L-Arginine | C6H14N4O2 | 173.1044 | [M-H]- | -0.1 | 131.0826, 156.0732, 68.9956 |
| P14 | 8.34 | LysoPC(14:0) | C22H46NO7P | 512.2994 | [M+HCOO]- | -0.8 | 452.2784, 227.2017, 168.0430, 78.9592 |
| P15 | 12.12 | LysoPC(16:0) | C24H50NO7P | 494.3252 | [M-H]- | -0.8 | 462.2978, 421.2724, 269.2484, 224.0689, 78.9593 |
| P16 | 9.81 | LysoPE(22:4) | C27H48NO7P | 528.309 6 | [M-H]- | -0.8 | 483.2499, 457.2362, 303.2331, 259.2429, |
| P17 | 3.35 | 3-Indoxyl sulfate | C8H7NO4S | 212.0023 | [M-H]- | -1.9 | 132.0454, 80.9655 |
| P18 | 4.83 | 2-Phenylethanol glucuronide | C14H18O7 | 297.098 | [M-H]- | -0.7 | 204.0664, 175.0243, 121.0661 |
| P19 | 9.49 | Phyllanthusol B | C35H49NO18 | 816.2932 | [M+HCOO]- | -1.5 | 628.2337, 507.2115, 346.1273, 168.0429 |
| P20 | 10.33 | LysoPC(20:3) | C28H52NO7P | 590.3463 | [M+HCOO]- | -1.4 | 530.3249, 460.2833, 305.2486 |
| P21 | 11.99 | LysoPC(22:4) | C30H54NO7P | 616.362 | [M+HCOO]- | 0.2 | 556.3398, 331.2634 |
| P22 | 14.11 | PI(18:0/0:0) | C27H53O12P | 599.3202 | [M-H]- | 0.3 | 419.2563, 315.0487, 241.0117, 152.8859, 78.9592 |
| P23 | 17.62 | dipalmitoylphosphatidic acid | C35H69O8P | 629.4552 | [M-H2O-H]- | -0.2 | 629.4542 |
| P24 | 11.19 | LysoPC(22:5) | C30H52NO7P | 614.3463 | [M+HCOO]- | -2.8 | 554.3245, 497.2881, 329.2483, 285.2598 |
| P25 | 7.12 | SM(d18:1/20:0) | C43H87N2O6P | 837.5491 | [M+Br]- | 1.0 | 429.2628, 407.2809, 251.2020, 195.1758 |
| P26 | 3.48 | L-DOPA | C9H11NO4 | 178.051 | [M-H2O-H]- | -1.1 | 178.0507, 134.0613 |
| P27 | 2.65 | L-Tryptophan | C11H12N2O2 | 205.0972 | [M+H]+ | -3.5 | 159.0952, 144.0831, 118.0683, 90.0482, 75.0249 |
| P28 | 16.63 | Linolenic acid | C18H30O2 | 279.2397 | [M+H]+ | -5.3 | 261.2286 |
| P29 | 18.04 | Linoleic acid | C18H32O2 | 263.2443 | [M-H20+H]+ | -0.6 | 263.2463, 121.1044 |
| P30 | 1.94 | L-Phenylalanine | C9H11NO2 | 166.0909 | [M+H]+ | -0.7 | 119.9480, 103.0005 |
| P31 | 1.14 | L-Tyrosine | C9H11NO3 | 182.0859 | [M+H]+ | -0.6 | 135.8930, 95.0005 |
| P32 | 0.8 | L-Valine | C5H11NO2 | 118.0862 | [M+H]+ | -1.1 | 118.0862 |
| P33 | 1.07 | Nicotinamide | C6H6N2O | 123.0549 | [M+H]+ | -1.5 | 108.0439, 80.0490 |
| P34 | 5.43 | prolyl-threonine | C9H16N2O4 | 217.1261 | [M+H]+ | -0.7 | 102.0550 |
| P35 | 20.17 | 11-beta-hydroxyandro-sterone-3-glucuronide | C25H38O9 | 483.2654 | [M+H]+ | -1.6 | 289.2164, 177.0396 |
| P36 | 8.53 | 4,4-dimethyl-5a-cholesta-8,24-dien-3-b-ol | C29H50O | 437.3815 | [M+Na]+ | 0.0 | 463.2347, 203.1849, 133.1047, 105.0726 |
| P37 | 1.05 | Carbamoylphosphate | CH4NO5P | 141.9642 | [M+H]+ | -0.8 | 98.9847 |
| P38 | 1.96 | Citric acid | C6H8O7 | 210.0590 | [M+NH4]+ | -0.8 | 193.0348, 133.0133 |
| P39 | 11.43 | 7-Dehydrodesmosterol | C27H42O | 400.3555 | [M+NH4]+ | -0.8 | 365.3203 |
| P40 | 9.98 | 11-hydroxyprogesterone 3-glucuronide | C27H38O9 | 507.2661 | [M+H]+ | -1.9 | 331.2273, 177.0395 |