Figure 4.

Quercetin enhanced the expression of FK506 binding protein 5 (Fkbp5) after corticosterone (CORT) treatment in cortical cells. After 24-hour exposure to (A–F) 5 µM xanthohumol or (G–L) 3 µM quercetin, cortical cells were treated for 96 hours with 200 µM CORT. The gene expression of Btg1, Fkbp5, Foxo3, Gilz, Nr3c1, and Sgk1 was quantitatively measured using real time reverse transcription polymerase chain reaction (RT-PCR). Results are expressed as the mean ± SEM of 3 independent experiments performed in triplicate (***P < .001; **P < .01; *P < .05 vs vehicle groups; #P < .05 vs CORT groups). Btg1, B-cell translocation gene 1; Foxo3, forkhead box O3; Gilz, glucocorticoid-induced leucine zipper; Nr3c1, nuclear receptor subfamily 3 group C member 1; Sgk1, serum and glucocorticoid-regulated kinase 1.