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Specificity of mouse anti-human PD-1 mAbs by western blot analysis. The recombinant PD-1 protein (40 kDa) was subjected to SDS-PAGE and blotted with the 1C4, 1H8, 2C4, 2H7, 3E5, 5B2, 5H7, 7C9, 7E5, 7G12, 9A5, 9B4, 9E11 and B1C4 mAb clones. PD-1, programmed cell death-1; mAb, monoclonal antibody.
