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Reconstitution of hexapeptide extension using separated modules (A–PCP–E–C architecture) from teicoplanin biosynthesis M5–M6 (Tcp11), different amino acid substrates for module 6, and the optional inclusion of the diiron monooxygenase enzyme CmlA from chloramphenicol biosynthesis. A, adenylation domain; C, condensation domain; PCP, peptidyl carrier protein domain; E, epimerization domain.
