Figure 4.

LCMS analysis of the reconstitution of hexapeptide extension using the separated M5/M6 modules from teicoplanin biosynthesis and different substrates for module 6 in the presence of CmlA. Peptide biosynthesis and CmlA-mediated hydroxylation reconstituted using tyrosine (A), Cl-Tyr (B), Br-Tyr (C), I-Tyr (D), F-Tyr (E), m-Tyr (F), Phe (G), and 3,5-di-Cl-Tyr (H) as M6 substrates. Solid lines indicate methylamide peptides (PCP-bound); dashed lines indicate hydrolyzed peptides (pentapeptide, black line; hexapeptides: pink line (Tyr), orange line (Cl-Tyr), green line (Br-Tyr), red line (I-Tyr), blue line (F-Tyr), pale pink line (m-Tyr), pale blue line (Phe) or purple line (3,5-di-Cl-Tyr)).