Table 1. Effect of Alternative Reductant/Mediator Combinations to Reconstitute CmlA Activity Using Tyr As a Substrate.
reductant | redox coupler | conversion, %a |
---|---|---|
NADH | phenazine methosulfate | 56 ± 7 |
NADH | methyl viologen | 61 ± 8 |
Dithionite | methyl viologen | N/D |
NADH | 24 ± 4 |
Triplicate experiments.