Table 2. Substrate Acceptance of CmlA Using a Coupled M5/M6 Teicoplanin Peptide Extension Assay.

substrate	A-domain rate (vs Tyr), %a	hexapeptide yield, %a
Tyr	100b	56 ± 7
F-Tyr	77 ± 12b	65 ± 6
Cl-Tyr	56 ± 13b	55 ± 8
Br-Tyr	61 ± 7b	37 ± 9
I-Tyr	59 ± 10b	46 ± 6
di-Cl-Tyr	63 ± 2b	37 ± 4
di-Br-Tyr	29 ± 6b	N/D
di-I-Tyr	89 ± 17b	N/D (peptide from I-Tyr only)
Meta-Tyr	31 ± 2	69 ± 7
Homo-Tyr	13 ± 10 (detection limit)	N/D
Phe	68 ± 10	84 ± 4
β-OH Phec	25 ± 9	86 ± 6

^aTriplicate experiments.

^bOriginal data from Kaniusaite et al.¹⁷

^cControl testing acceptance of β-OH Phe by M6 A-domain; no CmlA present.