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Determination of HBsAg-specific antibody titers and splenocyte cytokines. (A-D) The production of anti-HBsAg IgG (A), IgG1(B), IgG2a (C) and IgG2b (D) antibodies in plasma on day 21 were detected using ELISA. The secretion of IL-5 (E) and IFN-γ (F) in the splenocyte supernatants were detected using ELISA.
