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. 2020 Jan 1;10(2):657–670. doi: 10.7150/thno.39132

Figure 5.

Figure 5

Vaccine promoting antigen uptake by BMDCs. (A) BMDCs were incubated with soluble OVA-Rhod (2.5 µg mL-1), Vac-1 (Comp. 1/OVA = 10:1, w/w), or Vac-2 (Comp. 2/OVA = 10:1, w/w) at 37 °C for 1 h, stained with LysoTracker and Hoechst dye, and evaluated by confocal laser scanning microscopy. Magnification: 63 ×. (B) The percentage of OVA-Rhod-positive cells is shown. (C) Total fluorescence intensity of OVA-Rhod was measured; total fluorescence intensity (FI) = % of positive cells × mean fluorescence intensity. The bars shown represent the mean ± SE, and differences among groups were determined using one-way ANOVA. The asterisks indicate the significance of differences between the Vac-1 group and the indicated group. *: p < 0.05, **: p < 0.01.