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. 2020 Jan 1;10(2):725–740. doi: 10.7150/thno.39167

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Masson staining and CD31 immunochemistry staining of calvarial defects at 9-week post-surgery. (A) Masson staining of newly formed bone in the margin and center of the calvarial defect. Scale bar: 100 μm. L, left defect; R, right defect; NB, new bone; S, scaffold. The black arrows indicated the new vessels within the scaffolds. (B) Immunohistochemical staining for CD31 expression. The blue arrows indicated the CD31 positive staining around the vessel. Scale bar: 50 μm. C, control; P, PCL/nHA; HP, PCL/nHA+HPCH.