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. 2020 Jan 1;10(2):797–815. doi: 10.7150/thno.38483

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14-3-3ζ activates mitophagy gene expressions for neuronal survival. (A) Mitochondrial depolarization was inhibited by PTA using JC-1 staining assay. (B) Loss of mitochondrial mass was inhibited by PTA using MitoTracker Red FM staining assay. (C) Transmission electron microscope (TEM) analysis of mitophagy in Neuro-2A cells and PC12 cells. (D) PTA promoted colocalization of GFP-tagged LC3B (green) with MitoTracker (red). (E) PTA promoted colocalization of LysoTracker (red) with MitoTracker (green). (F) PTA-induced colocalization of GFP-tagged LC3B (green) with MitoTracker (red) was blocked in 14-3-3ζ siRNA-transfected HEK293T cells. (G) PTA-induced colocalization of LysoTracker (red) with MitoTracker (green) was blocked in 14-3-3ζ siRNA-transfected Neuro-2A cells. (H) PTA-induced inhibition of mitochondrial depolarization was blocked in 14-3-3ζ siRNA-transfected Neuro-2A cells. (I) PTA-induced inhibition of mitochondrial mass loss was blocked in 14-3-3ζ siRNA-transfected Neuro-2A cells. (J) PTA-induced inhibition of mitochondrial superoxide production was blocked in 14-3-3ζ siRNA-transfected Neuro-2A cells. Scale bars = 10 μm.