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. 2020 Jan 1;10(2):553–566. doi: 10.7150/thno.37181

Figure 2.

Figure 2

Mfn1 inhibits mitochondrial fragmentation and hypertrophy in cardiomyocytes. (A) Cardiomyocytes were exposed to ISO (10 μM) for the indicated time and the level of Mfn1 protein was detected by immunoblot. (B) Cardiomyocytes were infected with adenoviruses harboring Mfn1 or β-gal for 24 h, then incubated with ISO for additional 24 h. The expression level of Mfn1 protein was detected by immunoblot. (C and D) Mfn1 inhibits mitochondrial fission in hypertrophied cardiomyocytes. Cardiomyocytes were treated as described in (B). (C) Representative confocal images showing the level of mitochondrial fission in cardiomyocytes. (D) Quantitative analysis of the percentage of cells with mitochondrial fragmentation. (E-G) Mfn1 inhibits ISO induced hypertrophy in cardiomyocytes. Cardiomyocytes were treated as described in (B). Representative images of sarcomere organization in cardiomyocytes (E, left panel). Bar = 10 µm. Quantitative analysis of cell surface area (E, right panel). The mRNA levels of ANP (F) and β-MHC (G) were detected by qRT-PCR. *p < 0.05.