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. 2020 Jan 1;10(2):585–601. doi: 10.7150/thno.36572

Figure 2.

Figure 2

Cellular characterization of liposome uptake after one week of treatment. (A) DiD-labeled liposomes were injected intravenously into male ob/ob mice three times over the course of seven days. (B) Z-stack images of liver sections from ob/ob mice treated with tesaglitazar-loaded liposomes were stained with CLECS13F to identify Kupffer cells and assessed for co-localization with DiD-labeled liposomes. Co-localization of CLECS13F+ cells and DiD are marked by white boxes. (C) Peritoneal lavages and Epid and SC AT were harvested to stain peritoneal cavity (PerC) cells and SVF cells, respectively, for analysis by flow cytometry. The percentage of CD45+F4/80+ macrophages that were DiD+ was quantified. (D) Z-stack images of whole mounted Epid AT from an ob/ob mouse treated with tesaglitazar-loaded liposomes was stained with CD68 to identify macrophages and assessed for co-localization with DiD-labeled liposomes. Co-localization of interstitial CD68+ cells and DiD are marked by white arrows. The white box delineates the area of the merged image that is enlarged (right-most panel). (E,F) DiD+ macrophages and other cell subsets were also quantified as a percent of total DiD+ cells in the Epid AT (E) and SC AT (F), n = 5 in each group. The cell subsets analyzed were macrophages (Mac, CD45+F4/80+), B cells (CD45+CD19+), T cells (CD45+CD3+), other CD45+ Cells (Other 45+, CD45+CD19-CD3-F4/80-), endothelial cells (EC, CD45-CD31+), and other CD45- cells (Other 45-, CD45-CD31-). Data represents the mean ± SD.