Figure 4.

CD163⁻ but not CD163⁺ MNPs promote Th17, Th17/Th1 and Th17/Th22 responses in an IL-1β-dependent manner in UC patients. CD4⁺ T cells isolated from inflamed UC colon were co-cultured with or without autologous mucosal CD163⁺ [n = 9] or CD163⁻ [n = 27] cells, in the absence or presence of αIL-1R [n = 6], αIL-1β [n = 5] or αIL-12p70 mAbs [n = 8], then stained for intracytoplasmic [a,b] IL-17/IFN-γ, as well as [c,d] IL-17/IL-22 expression. [a,c] Wilcoxon signed rank test; [b,d] Repeated measures ANOVA with Bonferroni’s multiple comparison post test.