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. Author manuscript; available in PMC: 2020 Sep 18.
Published in final edited form as: J Am Chem Soc. 2019 Sep 9;141(37):14486–14490. doi: 10.1021/jacs.9b04179

Figure 3.

Figure 3.

Effect of competitive antagonist and BA1 on signal duration of PTHR1 agonists. a.b. Post-washout cAMP production mediated by 1 nM PTH, 1, 4 or 6 in HEK293 cells stably expressing hPTHR1. c. Washout assay data from part a and b presented as area under curve (AUC); data for measurements with BA1 are included. Data represent mean ± s.e.m of n = 4 (PTH(1-34)-NH2 and 1), n = 3 (4 and 6) independent experiments. All the ligand activities are normalized to that of PTH(1-34)-NH2 at 1 nM. Statistically significant difference using one-way ANOVA (**** P < 0.0001, *** P < 0.001, ** P < 0.01).