Table 5.
Cytokine concentrations in T cell culture supernatants (pg/ml).
| Cytokine | Without activation | Activation + GM-CSF (ng/ml) |
||||
|---|---|---|---|---|---|---|
| 0 | 0.01 | 0.10 | 1.00 | 10.00 | ||
| IL-2 | <10 | 590.0 (121.0–869.5) |
580.0 (96.0–987.5) |
540.0 (106.0–1034.5) |
468.0* (244.0–1120.0) |
614.0* (272.0–1142.0) |
| INF-γ | <10 | 5094.0 (4039.8–9688.3) |
2652.0* (2290.0–5530.0) |
3312.0 (2524.0–7669.5) |
5764.0 (3941.5–9071.5) |
6276.0 (3716.0–9346.0) |
| IL-4 | <2 | 11.6 (5.4–29.5) |
7.1 (0.5–30.6) |
7.4 (2.4–31.5) |
8.8 (4.6–31.6) |
13.9* (10.8–30.8) |
| IL-10 | <5 | 620.6 (484.8–870.9) |
528.9 (452.7–668.4) |
509.4 (430.2–732.3) |
512.1 (366.9–934.8) |
452.4* (322.2–769.5) |
Notes: T cell activation was performed in the presence of MACSiBead particles conjugated with Abs against human CD2, CD3, and CD28. Data are presented as Median with the first and third quartiles (Q1-Q3); * p < 0.05 – as compared to cell culture supernatants derived from activated T cells cultured in the absence of GM-CSF.