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. 2019 Dec 18;10:2953. doi: 10.3389/fimmu.2019.02953

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Copyright © 2019 Li, Wei, Zhang, Chi and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ss-Raptor regulates innate immune response during bacterial infection. (A–D) Rockfish was infected with L. anguillarum (A,B) or M.luteus (C,D), mRNA expression of Ss-Raptor in PBL (A,C) and head-kidney (B,D) was examined by qPCR at 6, 12, 24, and 48 h post stimulation, n = 4. (E) Spleen lymphocytes untreated or treated with PMA plus ionomycin were analyzed by immunoblot analysis with antibodies as indicated. (F,G) Rockfish was untreated or treated with rapamycin after L.anguillarum stimulation. The mRNA level of IL-1β (F) and IL-8 (G) were detected by qPCR (n = 4) *p < 0.05, **p < 0.01, ***p < 0.001.