Table 1.
Comparison of various approaches to DUB ABP cellular permeability with respect to the requirements for an ideal DUB ABP.
| ABP | Methods of cell entry | Requirements for an ideal DUB ABP | ||
|---|---|---|---|---|
|
Proteins captured in their native environment? (Yes/No/Partial) |
ABP labels a large spectrum (>10) of DUBs (Yes/No/Partial) |
Can use low (<1 μM) con-centrations of ABP? (Yes/No/Partial) |
||
| Traditional Ubiquitin-based e.g., HA-Ub-VME |
None | No | Yes | No |
| Pore-forming toxins | Partial | Yes | No | |
| Electroporation | Partial | Partial | No | |
| Cell-penetrating peptides | Partial | Yes | No | |
| Small molecules | Passive diffusion | Yes | Partial | Yes |