Table 2.
Primer sequences for quantitative PCR.
| Region | Gene symbol | Primers (5′-3′) | Accession No. |
|---|---|---|---|
| Distal | STAT5A | F: CATCACCATTGCCTGGAAGTT R: CCTTTCACCACGCGGG |
NM_214290.1 |
| VCAN | F: CAGGATACAGTGGCGACCAA R: CACATGCGTTGACGGTTTTAA |
NM_001206429.1 | |
| HOXB4 | F: CTCAAACTATGTCGACCCCAAGT R: CGATTACCTACCCAGCGACC |
ENSSSCT00000019089 | |
| SGPL1 | F: CACAGGAATGGGTGCGATCT R: CAGAATTGTCCTCCTTCTTCTTGG |
XM_003133063.4 | |
| RPL27A | F: GTCAGTGAGCAGACACGGATAAA R: CCTATCATTGACGTGGTGCG |
XM_005667051.2 | |
| TMEM200A | F: AGCCTGGATTAGGTGCAAAGA R: TGAGATTTGTTCCTCAAGCGTG |
XM_001927883.6 | |
| GBP1 | F: CCAGATGACCGCCAGCTAGA R: TGAGTCCGGCACGAGTTTG |
NM_001128473.1 | |
| Proximal | GNAS | F: AGCGCTCCAACGAGTACCA R: GATTGACGTCATCAAGCAGGC |
NM_001130215.2 |
| PDGFA | F: CTGGCTGGTGGCATCCAT R: CAGAAGTCATATCAAGCTGTAGGCTG |
XM_013987658.1 | |
| DDX20 | F: GGGACAGCCTCTGAGTGGAA R: AAATCCAGGTAAAGGCAAGGC |
XM_001928544.4 | |
| EIF2B2 | F: TGCTGCGCCGGATCA R: TGATGGAACTGATCCGCAGA |
XM_003128668.4 | |
| TOR1A | F: TTTGACGAAATGGATAAGATGCA R: TACCTGGACTATTACGACAACCTGG |
NM_001105298.1 | |
| KCNIP2 | F: TGAACTGGGCCTTCAACTTGT R: AAGGAGGAAATGCTCGACATCA |
XM_001928123.3 | |
| RPL27A | F: GTCAGTGAGCAGACACGGATAAA R: CCTATCATTGACGTGGTGCG |
XM_005667051.2 | |
| DIRAS3 | F: TCTACTCCGTCACCAAGAAGCA R: ATCTGATCCGCGAGCTCAA |
NM_001044598.2 | |
| Ref. gene | GAPDH | F: AGTCACAGCCCCAACTCGAT R: TTCCAGTTTCCATCCCAGACC |
ENSSSCT00000000756 |