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. 2019 Dec 18;10:2908. doi: 10.3389/fimmu.2019.02908

Figure 2.

Figure 2

Effect of UV-A treatment on lymphocytes population. Untreated PBMCs and UV-A-irradiated cells (Apo-cells) from the same donor were phenotypically characterized after 16 h of incubation. (A) Lymphocytes populations were analyzed and surface-stained using CD3+ CD4+/CD8 for T cells, CD19+ for B cells, and CD56+ for NK cells and myeloid cells based on FSC-SSC size (mean ± SEM) n = 4–6 independent experiments. (B) HLA-DR mean fluorescence intensity (MFI) was assessed on B cells and monocytes (n = 6 independent experiments). Statistics: paired Student t-test (*p < 0.05, **p < 0.01, ***p < 0.001). (C) Activation markers CD25 and CD69 were analyzed on CD4 T cells; T-reg population was described by activation markers CD127lowCD25highFoxp3+ (n = 3–4 independent experiments). Statistics: paired Student t-test (*p < 0.05, **p < 0.01).