Skip to main content
. 2019 Dec 18;10:2908. doi: 10.3389/fimmu.2019.02908

Figure 5.

Figure 5

Apo-cells as a tool to modulate allogeneic immune response. (A) Experimental scheme. PBMCs were cultured for 5 days in the presence of either allogeneic Apo-cells or irradiated PBMCs. At day 5, T cells were sorted out by flow cytometry, CFSE-labeled, and cultured in the presence of allogeneic irradiated PBMCs from the same donor or from third-party donor. Cumulative individual and mean ± SEM data from six independent experiments. Statistics: one-way ANOVA with post-hoc (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). (B) In vivo, co-injection in NOD/SCID/γC (NSG) mice of either allogeneic CD2– cells (APC) or allogeneic Apo-cells with CD2+ cells (containing mainly T and NK cells). Five days later, mice received an additional dose of allogeneic CD2– (APC) cells from the same source used for the first stimulation. 5-Ethynyl-2′-deoxyuridine (EdU) was injected at days 10 and 11 (2 h before sacrifice). Top right, percentages and numbers of human CD3+ cells at day 11 in the spleen of mice (CD3+ in huCD45+). Bottom left, CD4 and CD8 percentages in CD3+ cells, and percentages of EdU+ in CD4 and CD8 cells. Data represent two independent experiments, each with three mice per group. Statistics: unpaired Student t-test (*p < 0.05, **p < 0.01, ***p < 0.001).