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. 2019 Dec 24;9:19771. doi: 10.1038/s41598-019-56337-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of calibration equation between AM and mean of five MM mEZi quantifications for a second group of 33 images. (A) AM quantifications were compensated by the regression line equation that had been established in the initial calibration study, and were then compared with the mean of five repeated MM quantifications for each image. The solid line represents the Deming regression result: AM = 1.004(MM) + 0.139, R² = 0.991. (B) Bland-Altman plot for 33 validation images. The dashed red lines represent the 95% limits of agreement between the AM and MM (−0.07 and + 0.37).